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吴建中 支晔 Bidmon H J
【摘要】 目的 探讨一氧化氮(NO)对大鼠脑皮质离子型谷氨酸受体(NMDA、AMPA、KA受体)和GABA受体的影响。 方法 将Wistar大鼠腹腔注射神经细胞结构型一氧化氮合酶抑制剂7-硝基吲唑，以氚标配体分别标记NMDA、AMPA、KA和GABAA受体，用图像分析仪对大鼠额区、顶区、后肢区、梨状区、压部后区和味觉区皮质内标记受体进行定量分析。 结果 实验组大鼠额区、后肢区和梨状区内NMDA、AMPA、KA受体和GABAA受体含量均显著增加；顶区皮质内NMDA、KA受体和GABAA受体增加显著；味觉区皮质内KA受体增加显著。 结论 NO可能参与大鼠脑皮质离子型谷氨酸受体和GABA受体水平的调节。
【关键词】 7-硝基吲唑；受体；放射自显影；脑皮质；大鼠
【中图分类号】 R322.81 【文献标识码】 A 【文章编号】 0529-1356(2000)03-265
EFFECT OF NITRIC OXIDE SYNTHASE INHIBITOR
7-NITROINDAZOLE ON IONOTROPIC GLUTAMATE
RECEPTOR AND GABAA RECEPTOR
IN CEREBRAL CORTEX OF THE RAT
WU Jian-zhong ZHI Ye Bidmon H J
Department of Anatomy,North China Coal Medical College,Tangshan 063000,China
Department of Neuroanatomy,Heinrich-Heine University,40001 Dü sseldorf,Germany
【Abstract】 Objective To investigate the effects of nitric oxide (NO) on ionotropic glutamate receptors and GABA receptors in cerebral cortex of the rat. Methods The animals were injected with neuronal nitric oxide synthase inhibitor 7-nitroindazole(30 mg/kg,i p).The receptors in cerebral cortex were bound with［3H］MK-801 for NMDA receptors(NMDAR),［3H］AMPA for AMPA receptors(AMPAR),［3H］Kainate for kainate receptors(KAR) and ［3H］ muscimol for GABAA receptors(GABAAR),respectively,and quantitative analysis was used to study the changes of the receptors in some cortical areas.Results Six hours after the injection,NMDAR,AMPAR,KAR and GABAAR in frontal cortex(Fr),hindlimb area(HL) and prepiriform cortex(Pir)were markedly increased as compared with that of the control rats.In parietal cortex(Par) or in gustatory cortex(Gu),NMDAR,KAR,GABAAR or KAR were markedly increased respectively. Conclusion NO might be related to the regulation of ionotropic glutamate receptors and GABA receptors in cerebral cortex of the rat.
【Key words】 7-nitroindazole;Receptor;Autoradiography;Cerebral cortex;Rat
近年发现，一氧化氮(NO)是具有重要生理和病理功能的信息分子[1] [2] [3] 下一页
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