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屋尘螨对哮喘患者白细胞介素 |
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宋满景 李建强 杨变转 刘卓拉 张永兴
【摘要】 目的 探讨白细胞介素（IL）-10与支气管哮喘发病的关系，并对其释放影响因素进行研究。方法 对20例过敏性支气管哮喘患者和20例正常人外周血单个核细胞(PBLC)，分别在有无屋尘螨（HDM）和氟美松作用下进行体外培养，采用双抗体夹心酶联免疫吸附实验法对培养上清液中IL-10进行测定。结果 (1)哮喘患者PBLC培养液中IL-10浓度为(44.6±2.5) ng/L，正常对照为(53.6±8.2) ng/L，两组比较差异有显著性(P<0.01)。(2)加入HDM后IL-10分泌进一步受到抑制，而正常对照无明显变化。(3)在哮喘组加入氟美松后IL-10释放显著增加，正常对照也明显增加。结论 PBLC释放IL-10减少与哮喘发病有关，HDM和氟美松对IL-10的释放有影响。
【关键词】 哮喘 白细胞介素-10 螨 氟美松
A study on the effect of house dust mite on inteleukin-10 secretion of peripheral blood lymphocytes in patients with allergic asthma SONG Manjing, LI Jianqiang, YANG Bianzhuan, et al. Division of Respiratory Medicine, The Second Hospital, Shanxi Medical University, Taiyuan 030001
【Abstract】 Objective To investigate the relationship between interleukin (IL)-10 and allergic asthma, and the effect of antigen on IL-10. Methods The supernatant of cultured peripherial blood lymphocytes (PBLC) from 20 patients with allergic asthma and 20 normal subjects with defferent stimuli ［home-dust-mite (HDM) or dexamethasone］ was determined for IL-10, by means f enzyme-linked immunosorbent assay. Results (1) IL-10 was significantly decreased in asthma group, as compared with the control group (P<0.01); (2) Less IL-10 was released in HDM-stimulated PBLC in asthma (P<0.01); (3) IL-10 was obviously increased in dexamethason-stimulated whether in the asthma or in the control group. Conclusion The diminished IL-10 production from PBLC could be related to asthmatic attack in patients with allergic asthma. Dexamethason might exert, at least, a part of anti-inflammatory activity by inducing IL-10 secretion of PBLC.
【Key words】 Asthma Interleukin-10 Mites Flamethasone
外周血单个核细胞（PBLC）释放的细胞因子与过敏性支气管哮喘发病密切相关。白细胞介素(IL)-10作为一种细胞因子合成抑制因子，对哮喘的转归有重要影响［1，2］。本组对哮喘患者的PBLC进行培养，观察IL-10分泌水平及屋尘螨(HDM)和氟美松对其的影响。
对象与方法
一、对象
哮喘组20例，其中男11例，女9例，年龄16～46岁，平均(30±12)岁,病程(7.7±3.9)年,[1] [2] 下一页
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