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慢性乙型肝炎病毒感染者外周血T辅助细胞1 2型因子的表达 |
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姜荣龙 卢桥生 骆抗先 富宁
【摘要】目的 了解慢性乙型肝炎病毒(HBV)感染者外周血单个核细胞(PBMC)中CD4+T细胞内T辅助细胞1、 2型(Th1/Th2)因子、干扰素-γ(IFN-γ)和白细胞介素-4(IL-4)的表达情况，以测定Th0 /Th1/Th2细胞 的百分数，探明Th1/Th2细胞在慢性HBV感染中的作用。方法 常规分离PBMC，在佛波二酯(PMA)、Ionomycin、Monensin的刺激下 ，采用流式细胞检测仪(FACS)对慢性HBV感染者外周血单个CD4+T细胞内IL-4和IFN-γ表 达进行分析。结果 正常对照组，2.3%～18.6%的CD4+T细胞为Th1细胞，0.9%～9.2%为Th2细胞；在慢性HBV感染者外周血单个CD4+T细胞中，以Th0细胞居多；而Th1细胞则随 着慢性乙型肝炎肝脏炎症活动的加剧而明显增多，在炎症活动明显的慢性乙型肝炎，其Th1 百分数明显高于炎症活动呈静止状态的慢性乙型肝炎；Th2细胞在慢性HBV感染的不同阶段则 较为恒定，但明显高于对照组。结论 Th1细胞与炎症活动呈正相关，Th2细胞可能与HBV感染的慢性化相关 联。
【关键词】肝炎病毒，乙型；白细胞介素-4；干扰素-γ
Thelper 1,2 phenotype cytokines expression in perip heral blood of the patients with chronic hepatitis Bvirus Infection
JIANG Ronglong LU Qiaosheng LUO Kangxian et al.
（Department of Infectious Diseases, Nanfang Hospital, First Military Me dical University, Guangzhou 510515, China）
【Abstract】Objective The composition of T helper 0,1,2 cell(Th0/Th1/Th2) in chronic hepatitis B virve (HBV) infected individuals was investig ated by determining the expression of IL-4/IFN-γ in a single CD4+T cell iso late from periphesal blood mononuclear cell (PBMC), and the role of Th1/Th2 cy tokines in chronic HBV-infection was analyzed. Methods PBMCs from chronic HBV infected individuals were separa ted routinely, stimulated by phorboe-12-myristate-13-asetate (PMA)/Ionomyci n/Monensin, and the produ ction of interleukin-4 (IL-4) interferon-γ (INF-γ) by CD4+T cells in PBM C was determined by fluorescence activated call sorting (FACS) analysis. Results The percentage of IFN-γ-producing T cells and IL-4-p roducing T cells ranged from 2.3% to 18.6% and from 1.1% to 8.7% respectively in CD4+T cells from non-infected individuals. The majority of CD4+T cells fro m PBMCs in chronic HBV-infected individuals were Th0 cells. The percentage of T h1 cells increased significantly with the increasing of hepatic inflammation act ivity. The per centage of Th1 ce[1] [2] [3] 下一页
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