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孕酮从基因水平调节骨钙素的合成 |
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陈璐璐 余达林 Niels T. Foged
【摘要】 目的 观察孕激素对离体成骨细胞骨钙素基因表达及其合成的影响。方法 胎鼠头盖骨成骨细胞在含有不同浓度孕酮的培养基中培养约20天。培养不同时期的骨钙素mRNA含量及骨钙素的生成量通过Northern印迹杂交及放免的方法测定。结果 孕酮刺激骨钙素基因表达及其合成。其对mRNA表达的最大作用可达对照组的2倍以上。激素的作用呈时间及剂量依赖性。骨钙素mRNA表达及其生成的增加见于培养的中、晚期阶段，提示孕激素对成熟的成骨细胞更具潜能。此外，所选用的4种浓度的孕酮以浓度为10-6mol/L时作用最明显。结论 孕酮从基因水平调节离体胎鼠成骨细胞骨钙素的生成，这种调节呈现时间及剂量依赖。
【关键词】 孕酮 骨钙素 基因 成骨细胞
Progesterone increases osteocalcin production in vitro by regulating osteocalcin gene expression Chen Lulu,Yu Dalin,Niels T. Foged. Department of Endocrinology,Union Hospital,Tongji Medical University,Wuhan,430022
【Abstract】 Objective To investigate the effect of progestin on the gene expression and production of osteocalcin in vitro in fetal rat calvarial osteoblasts. Methods Fetal rat calvarial osteoblasts were cultured in the medium with different concentrations of progesterone for nearly 20 days. Osteocalcin mRNA and the amount of osteocalcin in the conditioned medium were determined by northern blot and RIA analysis at different periods of culture. Results Progesterone stimulated osteocalcin gene expression and production. In progesterone-treated cultures the maximal expression of gene was 2 times higher than the control. The effect of the hormone was time and dose-dependent. The amount of osteocalcin in the conditioned medium and the osteocalcin mRNA were increased in the middle and late periods of culture. In addition, 10-6mol/L progesterone acted more significantely on both gene expression and production of osteocalcin than the rest three doses of hormone. Conclusion Progesterone was involved directly in osteocalcin production by regulating gene expression and this effect of progesterone was time and dose-dependent.
【Key words】 Progesterone Osteocalcin Gene Osteoblast
(Chin J Endocrinol Metab, 1998, 14:190～192)
骨钙素是一含有r-羧基谷氨酸的蛋白质，由成骨细胞生成。它是成熟骨骨基质中丰富的非胶原蛋白之一。尽管骨钙素的生理功能至今尚不清楚，但血清中骨钙素的水平直接反映骨转化中成骨的情况〔1〕。骨钙素的合成主要受几种钙调节[1] [2] 下一页
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