【摘要】 目的 探讨槲皮素对人卵巢癌HO-8910细胞增殖与凋亡的作用。方法 MTT 法测定细胞增殖情况;流式细胞仪分析细胞周期及细胞凋亡;流式细胞仪检测槲皮素对凋亡相关蛋白Bcl-2、p53表达的影响。结果 槲皮素对HO-8910细胞有增殖抑制作用,呈时间、剂量依赖性;流式细胞术检测结果分析，实验组G1/G0期上升，S期和G2/M期下降，细胞凋亡率上升;流式细胞术结果显示，槲皮素使Bcl-2表达下调，而上调p53蛋白的表达。结论 槲皮素在体外能抑制卵巢癌HO-8910细胞的生长，阻止细胞由G1/G0期向S期和G2/M期移行并诱导细胞凋亡，其诱导HO-8910细胞凋亡的机制可能与上调促调亡蛋白p53表达和下调凋亡抑制蛋白Bcl-2表达有关。
【关键词】 槲皮素 卵巢癌 增殖 凋亡
The Effect of Quercetin on Proliferation and Apoptosis of HO-8910 Ovarian Cancer Cell
TANG Yan
Department of Obstetrics and Gynaecology，Jinzhou Central Hospital, Jinzhou 121001 China
Abstract： Objective To study the effect of quercetin on proliferation and apoptosis of HO-8910 ovarian cancer cell in vitro.Methods The proliferation of cells was detected by MTT method. Cell cycle and apoptotic percentage were analyzed by flow Cytometry (FCM). The expression of p53 and Bcl-2 were analyzed by FCM. Results Quercetin could inhibit the proliferation of HO-8910 cell as direct relation of concentrations and times. The flow cytometry showed that the proportion of G1 phase and the apoptotic rate were increased significantly in quercetin groups, the proportion of S phase and G2/M phase was reduced. In the FCM quercetin induced upregulation expression of p53 and downregulation expression of Bcl-2. Conclusions Quercetin could inhibit growth and induce apoptosis of ovarian cancer HO-8910 cell. Cell cycle was controlled in G1/G0.Meanwhile, quercetin could induce upregulation expression of p53 and downregulation expression of Bcl-2, while may be the mechanism of apoptosis of ovarian cancer by quercetin.
Key words：quercetin; ovarian cancer; cell proliferation;apoptosis
槲皮素(quercetin)是一种天然的黄酮类化合物，广泛分布于多种植物的花、果实、叶中，具有明确的抗感染、抗血小板聚集、抗氧化作用，并影响多种酶的活性。近年来其抗肿瘤作用逐渐为人们所认识并引起广泛的重视[1]。卵巢癌是女性生殖器官常见的恶性肿瘤之一，其致死率居妇科肿瘤的首位，在药物治疗上主要以化疗药物为主，这些药物在杀伤肿瘤细胞的同时，也会影响正常细胞的代谢，副作用较大，因此有必要寻找副作用小，疗效好，价格低廉的药物治疗卵巢癌，本实验观察了槲皮素对人卵巢癌HO-8910细胞增殖和凋亡的影响，并初步探讨其抗癌机理，为槲皮素应用于临床治疗提供实验依据。
1 材料与方法
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