C5a对中性粒细胞和肾小球内皮细胞粘附行为的影响

齐　洁　朱锡华

　　中国图书分类号　R371.3
　　摘　要　目的：为揭示C5a和C5aR对肾小球内皮细胞(GVEC)-中性粒细胞(PMN)粘附的影响及其影响程度。方法：采用免疫组化胶体金银染色检测C5aR在人GVEC上的表达，并用微管吸吮技术定量测定了经C5a诱导后，GVEC和PMN细胞对间的粘附力，同时通过测定粘附的PMN中髓过氧化物酶(MPO)含量变化，观察不同剂量C5a对粘附的影响。结果：发现GVEC上分布C5aR，经2 000 ng/ml C5a刺激，GVEC-PMN间粘附力(Fa)达0.014 dyn，相对粘附应力(S1)为1 720.0 dyn/cm2，与对照组Fa(0.001 dyn)和S1(339.9 dyn/cm2)均有非常显著差异(P＜0.01)。MPO测定结果表明GVEC-PMN粘附的发生依赖于C5a剂量。结论：从生物力学角度直接定量阐明了C5a-C5aR对GVEC-PMN粘附行为的重要影响。
　　关键词　C5a　C5a受体　肾小球内皮细胞　细胞粘附　微管吸吮技术

The effect of C5a on properties of adhesion between granulocytes and glomerulus vascular endothelial cells

QI Jie,ZHU Xi-Hua.
Department of Immunology,the Third Military Medical University,Chongqing 400038

　　Abstract　Objective：To reveal whether C5a and C5aR affect the adhesion between human glomerulus vascular endothelial cells(GVEC) and granulocytes for determining.Methods：Immunogolden-sliver staining the expression of C5aR on GVEC,micropipette aspiration technique quantitatively studying the degree of the adhesion between GVEC and PMN and by measuring the change of amount of myeloperoxidase(MPO) in adherent PMN for analysing the dose of C5a which made the adhesion.Results：C5aR was expressed on human GVEC,when treating with 2 000 ng/ml C5a,the adhesive forces (Fa) and relative adhesion stress(S1) respectively reach 0.014 dyn and 1 720.0 dyn/cm2,which had much markedly difference compared with that in controls(Fa=0.001 dyn,S1=339.9 dyn/cm2).The adhesion between GVEC and PMN was enhanced in a C5a dose dependent manner.Conclusion：The data strongly directly suggested that C5a plays a key role in the adhesion between GVEC and PMN.
　　Key words　C5a　C5a receptor　Glomerulus vascular endothelial cell　Cell adhesion　Micropipette aspiration technique

　　C5a作为体内重要的炎症介质和过敏毒素通过与靶细胞上的C5aR相互作用参与并介导了多种疾病的病理进程［1，2］。血管内皮细胞在炎症病变中最早受到损伤，已报道脐静脉内皮细胞，肺血管内皮细胞上分布C5aR，C5aR在肾小球内皮细胞(GVEC)上的分布还未见报道。本研究在发现GVEC表达C5aR的基 础上应用微管吸吮技术直接定量评价了 C5a和C5aR在 GVEC-PMN 粘附行为中的作用。

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