抗8种主要呼吸道病毒单抗杂交瘤细胞库的建立和临床应用

段佩若　张励力　韩洪彦　张容惠　赵　华　林　京　

　　中国图书分类号　R373.1
　　摘　要　目的：为了对流感病毒甲型和乙型、副流感病毒1、2和3型、呼吸道合胞病毒及腺病毒3、7型主要呼吸道病毒进行快诊。方法：从1986到1997年应用杂交瘤单抗技术，制备出以上4类8种抗病毒单抗细胞库，连同用于连接物的抗碱性磷酸酶单抗细胞株，共有117株单抗细胞株。将以上4类8种病毒单抗连接抗碱性磷酸酶单抗复合物，以兔抗鼠搭桥进行APAAP桥联快速诊断。结果：获得满意结果，此技术结果清晰，无内源性酶干扰，只需1台普通光学显微镜，2～3 h可获得准确结果。本系列快速诊断试剂已获得国家4项新药证书及5项生产文号。结论：此抗主要呼吸道病毒单抗细胞库，不仅可提供快诊试剂，也可提供病毒亚型分析、病毒变异以及基因表达研究试剂。
　　关键词　主要呼吸道病毒　单克隆抗体　杂交瘤细胞库

Establishment and clinical application of the hybridoma cell bank of monoclonal antibodies against 8 types of main respiratory viruses

DUAN Pei-Ruo, ZHANG Li-Li, HAN Hong-Yan et al.
Department of Clinical Experiment, No.262 Hospital of PLA, Beijing 100088

　　Abstract　Objective:The 8 types, 4 kinds of main respiratory viruses which cause human respiratory diseases, include influenza-A、B, parainfluenza virus 1、2、3, respiratory syncytial virus and adenovirus-3、7.Methods:Had developed a kit to rapidly diagnose these viruses from 1986 to 1997. By the technique of hybridoma, had prepared 117 hybridoma cell strains secreting McAbs of these 8 types, 4 kinds of viruses and of alkaline phosphatase. The McAbs form a junctional complex plus alkaline phosphatase anti-alkaline phosphatase (APAAP) bridged rapid diagnosis. Results：The result of the kit is credible, and no disturbance of Pendogenous enzyme. Performing the diagnosis, only need a general optical microscope and can finish in 2 or 3 h. The hybridoma cell bank can provide not only the reagents of the rapid diagnosis, but also the reagents for analysis of virus subgroups, virus variation and gene expression.Conclusion:The serial reagents of the rapid diagnosis have been granted 4 new drug certificates and 5 production certificates by the ministry of Health Department of National Health.
　　Key words　Main respiratory viruses　Monoclonal antibody　Hybridoma cell bank

　　国内外专著指出：对人类，尤其是婴幼儿，威胁最大的为流感病毒甲（Influenza-A,FluA）和乙型（Influenza-B,FluB）、副流感病毒1、2和3型（Parainfluenza virus 1、

[1] [2] 下一页