|
白细胞移动抑制因子 LMIF 释放的测定及条件性抑制LMIF的作用 |
| |
梅林 李乐群 范少光 丁桂凤
【摘要】 目的 选用与迟发过敏(DTH)反应密切相关的白细胞移动抑制因子(LMIF)的体外测定方法,分析在条件性免疫抑制反应中LMIF活性的变化。方法 LMIF测定是以间接毛细管方法结合分光光度计, 测定游出细胞的吸光度(A值)。在条件性反应训练和建立DTH反应模型的第7天,取耳称重,同时取小鼠肠系膜淋巴结细胞, 制备LMIF。结果 LMIF在条件反应组的作用明显减弱(A值升高), 与不经过条件性训练的对照组相比, 差异有非常显著意义(P<0.01, 方差分析, n=7), 但与非条件反应组相比没有差异,说明已形成了条件性抑制LMIF的反应。结论 本工作建立了稳定的LMIF吸光度检测法，建立了环磷酰胺条件性抑制LMIF的模型。为深入分析条件性免疫抑制反应的血清中的介导物质提供了一个方便的体外检测手段。
【主题词】 白细胞游走抑制因子 条件性免疫反应 2,4-二硝基氯苯
An assay of leukocyte migration inhibitory factor(LMIF) and the conditioned suppression effect on LMIF Mei Lin*, Li Lequn, Fan Shaoguang, et al. * Department of Physiology, Department of Immunology, Beijing Medical University, Beijing 100083
【Abstract 】Objective Our previous work showed that a conditioned immunosuppressive response could be induced in mice by following procedures: Camphor odor and intraperitoneal injection of cyclophosphamide were used as conditioned and nonconditioned stimulus, respectively. DTH response was induced by smearing 2,4-dinitrochlorobenzene (DNCB) on the abdominal skin (sensitization) and on the left ear (challenge) 5 days after the sensitization. Our previous work also showed that when the serum from the conditioned mice was injected into normal mice, DTH response was found to be suppressed significantly, indicating that a suppressive element existed in the conditioned serum. We in the present study want to build up a conditioned DTH response model in vitro so as to further analyze the property of this suppressive element. Methods With capillary incubation and EIA reader, the role of LMIF was judged by measuring the optical density (OD value) of migrated leukocyte. The procedures of conditioned response and DTH response were same as before except for the LMIF preparation. On the 7th day, the lymphocytes from the mice were stimulated with phytohemagglutinin(PHA) and then the OD value was measured. Results The OD value was increased both in conditioned group (P<0.01 as compa[1] [2] 下一页
上一个医学论文： 肺癌患者红细胞CR1基因密度型分布与红细胞免疫变化的研究
下一个医学论文： 异型反应性CD4 T淋巴细胞在MHC
|
|
|
|
|
|
|
您现在的位置： 绿色健康网 >> 医学论文 >> 基础医学论文 >> 正文