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矿物粉尘诱导的肺泡巨噬细胞因子对肺成纤维细胞作用的研究 |
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周建华1 周立人1 杨吉成2 陈跃进1 章瑜1 盛伟华2
【摘要】 目的 了解矿物粉尘诱导的肺泡巨噬细胞(AM)因子在肺纤维化中的作用。方法 用肺泡灌洗法获取兔AM，经体外培养,取其上清液,分别用同位素掺入法和四甲基偶氮唑盐比色法测定上清液中肿瘤坏死因子(TNF)和白细胞介素-6(IL-6)的活性,并将此上清液与人胚肺成纤维细胞(WI-38)培养，用氚-胸腺嘧啶(3H-TdR)、14碳-脯氨酸(14C-Pro)测定成纤维细胞增殖和胶原合成，用氯氨-T法测定总羟脯氨酸(HOP)含量。结果 石英、石棉和铀矿尘诱导的AM上清液均能促进肺成纤维细胞增殖和胶原合成，用3H-TdR掺入的每分钟计数(cpm)均值分别为6 584、3 848和6 893(100 μg浓度组)，14C-Pro掺入法cpm均值分别为27 952、13 416和18 538(200 μg浓度组)，明显高于对照组。AM上清液作用的肺成纤维细胞培养液总HOP含量分别为22.41、24.00和21.39 μg/ml(200 μg浓度组)，明显高于对照组(12.91 μg/ml)。石英、石棉和铀矿尘等矿物粉尘能刺激AM释放TNF和IL-6，其活性显著高于对照组，TNF活性分别为1 396、1 198和852 U/ml；IL-6活性分别为1 336、1 511和1 335 U/ml。抗TNF抗体和γ干扰素(IFNγ)能抑制肺成纤维细胞增殖，IFNγ的作用弱于抗TNF抗体，对胶原合成的抑制作用，IFNγ强于抗TNF抗体。结论 矿物粉尘所致的肺纤维化与TNF和IL-6的异常表达有关。抗TNF抗体和IFNγ能拮抗TNF和IL-6的作用。
【关键词】 矿物质 粉尘 巨噬细胞，肺泡
Studies on Effects of Cytokine Released from Alveolar Macrophage Induced by Mineral Dust on Lung Fibroblast Zhou Jianhua*, Zhou Liren, Yang Jicheng, et al. *Department of Occupational Health, Suzhou Medical College, Suzhou 215007
【Abstract】 Objective To understand the role of cytokine released from alveolar macrophage (AM) in lung fibrosis caused by mineral dust. Methods Rabbit′s AM obtained by lavage was cultured with mineral dust in vitro. Activities of tumor necrosis factor (TNF) and interleukin 6 (IL-6) in its supernatant were determined with isotope labelling method and MTT colorimetry, respectively. Human fetal lung fibroblast WI-138 was cultured with this supernatant. Proliferation of fibroblast and synthesis of collagen were examined by 3H-thymidine (3H-TdR) and 14C-proline (14C-Pro) incorporation and its total hydroxyproline (HOP) level was analyzed by chloramine-T method. Results Proliferation of lung fibroblast and synthesis of collagen could be enhanced by the supernatant containing AM induced by quartz, asbestos and uranium mineral dust, with 3H-TdR incorporated counts per minute (cpm) of 6 584, 3 848 and 6 893 in the group [1] [2] [3] 下一页
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