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大鼠肝内起始淋巴管超微结构观察 |
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赵玲辉 李玉兰 张莉 张雅芳 张云芳 王云祥
【摘要】 目的 观察大鼠肝内起始淋巴管(毛细淋巴管)的超微结构特点，并测量有关数据资料，探讨肝淋巴生成途径。 方法 半薄切片光镜观察，超薄切片透射电镜观察，计算机图像分析。 结果 肝起始淋巴管内皮细胞中富含溶酶体和质膜小泡，小泡的数密度为39.80个／μm3，体密度为0.028。小泡的平均直径为90.6nm。内皮细胞连接有3种类型，端端连接占18.7%，重叠连接占46.0%，嵌插连接占34.0%，处于开放状态的占1.3%。内皮细胞连接处有特殊连接装置的占60.7%。 结论 研究结果提示肝淋巴生成可能以小泡转运为主要途径。
【关键词】 起始淋巴管；超微结构；图像分析；肝；大鼠
【中图分类号】 R322.26 【文献标识码】 A 【文章编号】 0529-1356(2000)03-218
THE ULTRASTRUCTURE OF HEPATIC INITIAL
LYMPH VESSELS OF RAT
ZHAO Ling-hui，LI Yu-Lan,ZHANG Li，ZHANG Ya-fang ZHANG Yun-fang, WAGN Yun-xiang
(Department of Anatomy, Harbin Medical University, Harbin 150086，China)
【Abstract】 Objective To study the pathway of lymph formation in liver, the ultrastructures of hepatic initial lymph vessels of rat were observed and the relative data were measured. Methods The semithin sections were observed under light microscope, then the ultrathin sections were observed under transmission electron microscope and finally by means of computer image analysis system data were measured. Results A lot of lysosome and vesicles existed in endothelia of hepatic initial lymph vessels. Profil density(39.8 vesicles／μm3), volume density(0.028) and average diameter(90.6nm) of vesicles were measured. The type and opening rate(1.3%) of interendothelial contacts were determined. The type of the interendothelial contacts was end-end(18.7%)， overlapping (46.0%) and interdigitating(34.0%) junction. The interendothelial contacts with special junctional complexes was 60.7% or so. Conclusion The study showed that vesicles transport might be the main pathway of lymph formation in liver.
【Key words】 Initial lymph vessel; Ultrastructure; Liver; Rat
组织液及部分大分子物质进入淋巴管形成淋巴的途径有两条，即通过开放的内皮细胞连接和内皮细胞质中的小泡吞饮转运作用。在不同器官内淋巴生成的主要途径可能不同。如胃［1］等器官是以开放的内皮细胞连接为主要转运途径，有的器官则可能以小泡转运为主要途径。那么肝的淋巴生成是以哪条途径为主，Niiro［2］报道鼠肝淋巴生成途径是以小泡转运为主，Magari［3］又认为是以内皮细胞连接开放为主要途径。因此，研究肝起始淋巴管内皮细胞超微结构的特点，尤其是获得相关数据资料，将成为认识肝[1] [2] 下一页
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