|
表达疟原虫裂殖子表面蛋白1减毒鼠伤寒杆菌的稳定性 |
| |
钱锋 管惟滨 方军 蒋春雷
【摘要】 目的 检测含恶性疟原虫裂殖子表面蛋白1基因片断的重组减毒鼠伤寒杆菌X4064(pQEM1)的入侵宿主能力，所含质粒的稳定性和再表达能力。方法 用含恶性疟原虫裂殖子表面蛋白1第1号基因片段的重组减毒鼠伤寒杆菌X4064(pQEM1)灌胃接种BALB/c小鼠，用平板培养法、质粒酶切和免疫印迹反应，鉴定从小鼠组织中分离到的该重组菌，测定其表达能力，及在小鼠组织中的消长。结果 从小鼠组织中分离到的减毒鼠伤寒杆菌X4064株含有重组的pQEM1质粒；减毒鼠伤寒杆菌X4064(pQEM1)体外表达了恶性疟原虫裂殖子表面蛋白1第1号片断；X4064(pQEM1)在小鼠组织中的消长曲线与X4064的消长曲线基本一致。结论 重组pQEM1质粒能稳定地存在于减毒鼠伤寒杆菌X4064株中，不妨碍其入侵宿主细胞，从小鼠组织中分离到的X4064(pQEM1)依然具有表达M1蛋白的能力。
【关键词】 疟原虫，恶性 沙门氏菌，鼠伤寒
Expression and Stability of Fragment of Plasmodium Merozoite Major Surface Protein 1 in Recombinant Attenuated Salmonella Typhimurium QIAN Feng, GUAN Weibin, FANG Jun, et al. Department of Parasitology, The Second Military Medical University, Shanghai 200433
【Abstract】 Objective To determine the invasive ability of recombinant attenuated Salmonella typhimurium X4064（pQEM1）strain containing gene fragment of Plasmodium falciparium merozoite surface protein 1 （MSP1），the stability of its plasmid， and its re-expression. Methods BALB/c mice were fed with recombinant attenuated S. typhimurium containing No. 1 gene fragment of P. falciparium MSP1 by gastric tube．Plate incubation，plasmid endonuclease analysis and Western blot were used to identify the recombinant attenuated S. typhimurium strains isolated from mice and the ability of re-expression， and its growth were determined in vitro. Results The attenuated strain X4064 of S. typhimurium isolated from mice contained recombinant plasmid pQEM1，no. 1 MSP1 fragment of P. falciparium was expressed in vitro in S. typhimurium X4064（pQEM1）strain，and its growth curve of X4064（pQEM1）strain in mice was basically similar to that of X4064. Conclusion The recombinant plasmid pQEM1 could steadily exist in the X4064 strain of S. typhimurium，without influence on its invasion into host cells．X4060（pQEM1）strain isolated from infected mice still had the ability to re-express M1 protein.
【Key words】 Plasmodium falciparium Salmonella typhimurium
[1] [2] 下一页
上一个医学论文： 日本血吸虫卵计量变异的模型建立与参数估计
下一个医学论文： 职业与妊娠经过和生殖结局关系的探讨
|
|
|
|
|
|
|
您现在的位置： 绿色健康网 >> 医学论文 >> 基础医学论文 >> 正文