【摘要】 目的:探讨拉莫三嗪(LTG)对围生期缺氧导致惊厥的新生大鼠(生后10 d)的神经保护作用。方法：新生大鼠随机分成缺氧生理盐水组、缺氧LTG组、常氧生理盐水组和常氧LTG组4组。缺氧生理盐水组(n=26)：用梯度缺氧方法制作急性缺氧模型(氧浓度低于4%)，缺氧前0.5 h已腹腔注射生理盐水;缺氧LTG组(n=26)：腹腔注射LTG(20 mg·kg-1)，0.5 h后放入同上的缺氧装置;常氧生理盐水组(n=26)：腹腔注射生理盐水后放入常氧浓度的相同装置;常氧LTG组(n=26)：腹腔注射LTG后放入同上的常氧浓度装置。尼氏染色评估惊厥发作1 h后及生后30 d大鼠的细胞丢失情况，Timm染色观察生后30 d大鼠的苔藓纤维发芽情况，戊四氮测量生后30 d大鼠的惊厥敏感性。结果：尼氏染色结果显示各组大鼠在惊厥发作1 h及生后30 d的两个时间点均未见明显神经元丢失。缺氧LTG组在围生期缺氧惊厥的程度及敏感性与缺氧生理盐水组无明显差异，常氧两组均未见惊厥发作。但在生后30 d，缺氧LTG组大鼠对戊四氮的惊厥阈值较缺氧生理盐水组明显增高(P<0.05)，较常氧组降低(P<0.01)，常氧两组间无差异(P>0.05)。缺氧LTG组大鼠在生后30 d时，齿状回颗粒上层的苔藓纤维发芽分数较缺氧生理盐水组明显降低(P<0.05)，但与常氧两组间均无明显差异。结论：拉莫三嗪对围生期缺氧导致惊厥的大鼠有神经保护作用，降低了大鼠对化学致痫药物的敏感性，并且阻止了远期发展为癫痫的可能。
【关键词】 拉莫三嗪; 新生大鼠; 缺氧; 惊厥; 神经保护作用; 苔藓纤维发芽
Neuroprotective effects of lamotrigine on neonatal rats after
perinatal hypoxiainduced seizures
WANG Minghuan1, DENG Yan2, JIANG Li1, HE NongYue2(1. School of Clinical Medicine, Southeast University, Nanjing 210009, China; 2. State Key Laboratory
of Bioelectronics, Southeast University, Nanjing 210009, China)
[Abstract] Objective: To investigate the possible neuroprotective effects of lamotrigine(LTG) on neonatal rats after hypoxiainduced seizures at postnatal day 10. Methods: Four groups of rats were studied at postnatal day 10. Rats in hypoxianormal saline(NS) group(n=26) were subjected to acute hypoxia at postnatal day 10 according to the protocol of progressively decreasing oxygen(down to 4% O2) and intraperitoneally injected with NS half hour before hypoxia. Rats in hypoxiaLTG group(n=26) were subjected to acute hypoxia and received LTG(20 mg·kg-1) intraperitoneally half hour before hypoxia. Rats in normoxiaNS group(n=26) were injected with NS and placed in normal oxygen apparatus. NormoxiaLTG group(n=26) were injected with LTG and placed in normal oxygen apparatus. Nissl staining was performed to evaluate cell loss at one hour after onset of seizure and at postnatal day 30. Ti
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