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白细胞介素2基因转导对卵巢癌细胞系SKOV3免疫原性的影响 |
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张震宇 郎景和
【摘要】 目的 探讨白细胞介素2 (IL-2)基因转导对卵巢癌细胞系SKOV3免疫原性的影响。方法 应用流式细胞术，测定SKOV3细胞组织相容性白细胞抗原(HLA)HLA-ABC、HLA-DQ及HLA-DR分子的表达，51Cr-4h释放实验显示SKOV3、SKOV3/Neo、SKOV3/IL-2细胞对自然杀伤(NK)细胞和淋巴因子激活杀伤(LAK)细胞杀伤的敏感性。结果 SKOV3/IL-2细胞中HLA-ABC、 HLA-DQ 及HLA-DR分子的表达率(17.7%、19.1%及9.4%)较SKOV3(1.5%、2.4%及4.7%)和SKOV3/Neo(4.3%、6.9%及2.1%)显著提高；SKOV3、SKOV3/Neo和SKOV3/IL-2细胞对NK细胞的杀伤均不敏感，但SKOV3/IL-2细胞对LAK细胞的杀伤敏感性较SKOV3、SKOV3/Neo细胞提高约1倍，差异有非常显著性；肿瘤细胞与淋巴细胞混合培养显示，淋巴细胞有向SKOV3/IL-2细胞聚集的趋势。结论 IL-2基因转导可提高卵巢癌细胞系SKOV3的免疫原性。
【关键词】 卵巢肿瘤； 白细胞介素2；基因转移； 癌症疫苗； 肿瘤细胞，培养的
Effects of Human Interleukin-2 Gene Transfer on the Immunity of Ovarian Cancer Cell Line SKOV3
ZHANG Zhenyu, LANG Jinghe.
（Deptment of Obstetrics and Gynecology, General Hospital of Beijing Military Command, Beijing 100700, China）
【Abstract】 Objective To study the effect of interleukin-2 (IL-2) gene transfer on the immunity of ovarian cancer cell line SKOV3. Methods The expression of main histologic complex (MHC) molecular HLA-ABC, HLA-DR and HLA-DQ of SKOV3, SKOV3/Neo and SKOV3/IL-2 cells were detected by flow cytometry. The 51 Cr-4hr releasing test was employed to detect the killing effects of natural killer (NK) and lymphokine-activated killer (LAK) cells. Meanwhile, the tumor cell and lymphocyte co-culture was performed. Results The expression of HLA-ABC, HLA-DR and HLA-DQ molecular of hIL-2 gene modified cells was upregulated markedly. The 51Cr-4hr releasing test showed that the parental and gene modified cells were markedly resistant to NK cells, but the IL-2 gene modified cells was more sensitive to LAK cells. There were significant differences between them (P<0.01). The “cross-talk” of tumor cell with lymphocyte was observed when the hIL-2 gene modified SKOV3 cells were cocultured with lymphocyte. Conclusion IL-2 gene transfer can enhance the immunity of ovarian cancer cell line SKOV3.
【Key words】 Ovarian neoplasms; Interleukin-2; Gene transf[1] [2] 下一页
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