糖基化终产物对人单核细胞EMMPRIN基因表达的影响

【摘要】  目的：探讨糖基化终产物（AGEs）对人单核细胞细胞外基质金属蛋白酶诱导因子（EMMPRIN）表达的影响。方法：在培养的人单核细胞株中分别加入不同浓度AGEs干预24 h和同一浓度AGEs干预不同的时间点，以1640培养基和相应浓度的牛血清白蛋白为对照组。用逆转录聚合酶链反应检测EMMPRIN mRNA的表达水平。结果：AGEs干预人单核细胞的EMMPRIN mRNA水平与对照组相比差异有统计学意义（P＜0.05）；且其表达呈时间和浓度依赖性降低（P＜0.05）。结论：AGEs以时间及浓度依赖的方式减少人单核细胞EMMPRIN mRNA的表达。

【关键词】  糖基化终产物； 人单核细胞； 细胞外基质金属蛋白酶诱导因子； 逆转录聚合酶链反应； 糖尿病

Effect of advanced glycation end products on the gene expression of EMMPRIN in cultured

    human monocytes

    SUN Yeping， 1SUN Zilin， 1ZHOU Li      （The People’s Hospital of Jingjiang City， Jingjiang 214500；1Shcool of Clinical Medical Science，Southeast University）

    [Abstract]   Objective：To investigate the effects of advanced glycosylation end products （AGEs） on the expression of extracellular matrix metalloproteinase inducer（EMMPRIN） in cultured human monocytes. Methods：The cultured human monocytes were incubated with AGEs （50，100，200，400 mg·L-1） for 24 h or 200 mg·L-1 AGEs for 8、16、24 and 48 h respectively，which were incubated with 1640 medium and BSA as a control. The mRNA expression of EMMPRIN in human monocytes was analyzed by RT-PCR. Results：Compared with that in cells incubated with 1640 and BSA， the level of EMMPRIN mRNA in cells treated with AGEs decreased significantly in a concentration-dependent and time-dependent manner. Conclusion：The AGEs inhibit the expression of EMMPRIN in cultured human monocytes.

    [Key words]   Advanced glycation end products； Human monocytes； Extracelluar matrix metalloproteinase inducer；Reverse transcriptase polymerase chain reaction；Diabetes mellitus细胞外基质（extracellular matrix，ECM）

    在血管周围及其它组织的积聚是糖尿病慢性血管并发症的主要病理机制之一。细胞外基质金属蛋白酶诱导因子（extracellular matrix metalloproteinase inducer， EMMPRIN）能刺激细胞外基质金属蛋白酶（matrix metalloproteinase， MMPs）如

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