【摘要】 目的 通过动物实验初步探讨驱铅益智口服液(口服液)的安全性，旨在开发一种安全有效的驱铅产品。方法 依据《食品安全性毒理学评价程序和方法》的要求，采用霍恩氏法进行急性毒性试验，应用Ames试验、小鼠骨髓嗜多染红细胞微核试验、小鼠精子畸形试验和30 d喂养试验进行遗传毒性和安全性评价。结果 急性毒性试验显示小鼠经口的LD50>10 g·kg-1·bw-1;3种遗传毒性试验均显示口服液组与阴性对照组相比差异均无统计学意义 (P>0.05);30 d喂养试验期间大鼠一般状况、体重、食物利用率、外周血常规和生化指标、脏体比均与阴性对照组相比差异均无统计学意义 (P>0.05)。光镜下病理学检查组织结构均未见异常。结论 从食品毒理学角度出发，可以认为口服液无毒、无致突变作用。
【关键词】 铅中毒;安全性评价;毒性实验; 动物;实验室
Safety and toxicological assessment of Quqian Yizhi health oral liquid HAN Changcheng*,NIU Xiuli,SONG Bo*,et al.*Department of Nutrition and Food Hygiene,Public Health Collage of Hebei Medical University,Shijiazhuang 050017,China
【Abstract】 Objective To investgate the safety and toxity of Quqian Yizhi Health Oral Liquid (QYHOL) in order to develop an effective and safe, leadexpelling product to prevent and treat lead poisoning.Methods According to the requirements of the toxicological evaluation procedure and method of food safety,the acute toxicity test was used,genetic toxicity test including Ames test,bone marrow polychromatic erythrocyte tiny nuclear test and spermmutation test and thirtyday feeding test were performed to observe the safety of QYHOL.Results Data obtained from acute toxicity test indicated LD50 of the QYHOL was more than 10g·kg-1·bw-1, by gavage.Three genetic toxicity tests showed that there was no difference between QYHOL group and negative control group
(P>0.05).In thirtyday feeding test,no obvious changes were found for weight change,food utilization ratio,blood routine test and biochemical indexes,etc.All pathological sections in HE staining derived from rat liver,spleen,kidney and other organs did not reveal abnormal structures by light microscope.Conclusion QYHOL is a safe product, without toxicity and side effects.
【Key words】 lead poisoning;safety assessment;toxicity test; animal;laboratory
由于工农业生产和日常生活中含铅物质的广泛应用，铅污染已经成为危害人类健康的严重社会问题[1]。铅是一种有毒的重金属，当体内铅含量超过一定水平
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