【摘要】 目的 探讨电离辐射对胶质瘤C6细胞垂体瘤转化基因(pituitary tumor transforming gene， PTTG)表达的影响。方法 胶质瘤C6细胞分为4组(n=6)：A组(空白对照组)、B组(2 Gy剂量X射线组)、C组(4 Gy剂量X射线组)、D 组(6 Gy剂量X射线组)，X射线照射后12 h，逆转录聚合酶链反应(RT-PCR)检测PTTG mRNA的表达,免疫细胞化学检测其蛋白表达。结果 PTTG mRNA在X射线处理组均降低，随着X射线剂量的增加，其表达降低越明显，A、B、C、D 4组的表达分别为 1.910±0.141、 1.398±0.157、1.010±0.133、0.743±0.092，各组间差异均有统计学意义(P< 0.01)。PTTG蛋白积分光密度 (integrated optical density，IOD) 在X射线处理组均降低，随着X射线剂量的增加，其表达降低越明显，A、B、C、D 4组的IOD分别为1.840±0.089、1.395±0.139、0.893±0.125、 0.597±0.104，各组间差异均有统计学意义(P< 0.01)。结论 电离辐射可以剂量依赖的方式降低胶质瘤C6细胞PTTG mRNA及其蛋白的表达。
【关键词】 胶质瘤;电离辐射;垂体瘤转化基因
Effect of ionizing radiation on the expression of pituitary tumor
transforming gene in glioma C6 cells
JIANG Ping1, DAI Rufei2, CAI Jun2, LIU Ning3, LUO Hui3, YAN Zhihai2, YAN Chao2
(1. Department of Radiotherapy, The Second Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu 221006,
China;2. Department of Neurosurgery, The Second Affiliated Hospital of Xuzhou Medical College;
3. Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 221029)
Abstract: Objective To investigate the effect of ionizing radiation on the expression of pituitary tumor transforming gene (PTTG) in glioma C6 cells. Methods Glioma C6 cells were divided into 4 groups (n=6): group A (control group), group B (2 Gy doses of X-ray group), group C (4 Gy doses of X-ray group), group D (6 Gy doses of X-ray group). The expression of PTTG mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR) 12 hours after X-ray irradiation and the expression of PTTG protein was detected by immunostaining assay using streptavidin-peroxidase (SP) method. Results The expression of PTTG mRNA decreased in X-ray treatment groups, which was positively correlated with the increase of X-ray doses. The expression of PTTG mRNA in groups A, B, C and D were 1.910±0.141, 1.398±0.157, 1.010±0.133, 0.
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