细胞凋亡和P53在宫颈癌及宫颈上皮内瘤变中的表达

乔玉环　吴丹　史惠蓉　孙莹璞　苑中甫

　　【摘要】　目的：探讨细胞凋亡和P53在宫颈癌及宫颈上皮内瘤变中的作用。方法：用DNA缺口末端标记技术(TUNEL)和免疫组化技术原位观察15例正常宫颈、22例CIN?I/Ⅱ、16例CINⅢ和52例浸润性宫颈鳞癌(ISCC)组织中细胞凋亡标记指数(A-LI)、PCNA(增殖细胞核抗原)标记指数(PCNA-LI)和P53表达阳性率及P53标记指数(P53-LI)。结果：在正常宫颈上皮未检测到凋亡细胞，随着CIN病变的进展，A-LI增高(P＜0.05)，在ISCC，A-LI明显低于CIN(P＜0.01)；PCNA-LI随宫颈癌各级病变的进展逐渐增高(P＜0.05)；在正常宫颈和CINI/Ⅱ均未检测到P53表达，在CINⅢ，P53-LI明显低于ISCC(P＜0.01)。结论：细胞凋亡在宫颈癌发生发展过程中发挥重要作用，P53蛋白的积聚可能对细胞凋亡起抑制作用。
　　【关键词】　宫颈肿瘤；细胞凋亡；蛋白质，P53
　　中图分类号　R737.33

Apoptosis and expression of p53 gene in cervical intraepithelial neoplasia and squamous cell carcinoma

Qiao Yuhuan，Wu Dan，Shi Huirong
（The First Affiliated Hospital of Henan Medical University,Zhengzhou,450052）

　　Abstract objective：To explore the effects of apoptosis and p53 gene in cervical intraepithelial neoplasia and squamous cell carcinoma.Methods:The apoptotic labeling indices(A-LI),PCNA(proliferating cell nuclear antigen)-LI,P53-LI and the positive expression of P53 protein were observed in 15 cases of normal cervical epithelium,22 cases of CIN I/Ⅱ，16 cases of CINⅢ and 52 cases of ISCC(invasive squamous cervical carcinoma)tissues,using DNA nick end labeling technique and immunohistochemical staining for PCNA and P53 proteins.Results:No apoptotic cell was observed in normal cervical epithelium.A-LI increased with the development of CIN(P＜0.05).A-LI in ISCC were significantly lower than those in CIN(P＜0.01).PCNA-LI graduallincreased with the development of every lesion grade of cervical carcinomas(P＜0.05).P53 protein was not detected in normal cervical epithelium and CIN I/Ⅱand P53-LI in CINⅢ were significantly lower than those in ISCC(P＜0.01).Conclusions:Apoptosis play an important role in the development of cervical carcinoma and P53 protein may block apoptosis.
　　Key words　Cervical neoplasms;Apoptosis;Protein，P53
 
　　组织内环境的稳定依赖细胞增殖和死亡间的平衡，肿瘤发生和细胞不能适时发生凋亡密切相关［1］。p53是重要的细胞凋亡调控基因，了解宫颈癌细胞恶性转化过程中p53基因的变化及其与细胞凋亡的关系有助于揭示宫颈癌的发生机制。我们应用DNA缺口末端标记

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