肺心病患者红细胞膜脂质过氧化作用与红细胞免疫功能之间的关系

姜永堂　陈学魁　安继红　赫国志　

　　中国图书分类号　R541.5　R392.12
　　摘　要　目的：探讨肺心病患者脂质过氧化对红细胞免疫功能的影响。方法：采用红细胞酵母菌混合花环法和硫代巴比土酸(TBA)法检测了肺心病患者红细胞C3b受体、血浆及红细胞膜丙二醛(MDA)水平。结果：肺心病患者红细胞C3b受体明显降低；血浆MDA和红细胞膜MDA明显升高；在急性期肺心病患者上述变化更为显著，同时伴有红细胞免疫复合物花环率降低；红细胞C3b受体和血浆MDA及红细胞膜MDA呈负相关，多元逐步回归分析膜MDA和C3b受体降低关系更为密切。结论：红细胞膜脂质过氧化损害对红细胞C3b受体有不利影响。
　　关键词　肺心病　红细胞免疫功能　脂质过氧化

Relationship between peroxidation in erythrocyte membraneand and red cell immune function in cor pulmonale subjects

JIANG Yong-Tang,CHEN Xue-Kui,AN Ji-Hong et al.
Department of Respiratory System，The 2nd Clinic Colleage-Nornam Bethune University of Mecical Sciences,Changchun 130041

　　　Abstract　Objective：To evaluate the relationship between peroxidation in erythrocyte membrane and red cell immune function in cor pulaonale.Methods：The red cell immune function and the levels of malonyldialdehyde(MDA) in plasm and erythrocyte membrane were tested by red cell yeast mixture rose and thiobarbituric acid(TBA) methods.Results：The levels of erythrocyte C3b receptor was significantly decreased in acute stage cor pulmonale(P<0.01).The levels of plasma and erythrocyte membrane MDA in cor pulmonale were higher than those in healthy(P<0.01).Conclusion：The results indicated that lipid peroxidation,particularly in erythrocyte membrane,might contribute to the decrease of erythrocyte of receptor.
　　Key words　Cor pulmonale　Red cell immune function　Lipid peroxidation

　　丙二醛(MDA)是脂质过氧化反应中重要的中间产物，MDA可严重损坏细胞膜组分、结构和功能〔见：Jain S K.The accumulation of malonyldialdebyde,an end of product of fatty acid peroxidation ,can disturb aminophospholipid organization in the membrane bilayer of human erythrocyte.J Biol Chem,1984;259:339〕。红细胞膜上存在具有免疫粘附活性的C3b受体，由于红细胞免疫粘附(Red cell immune adherence,RCIA)作用，红细胞得以发挥携带和清除循环免疫复合物(CIC)，促进吞噬细胞吞噬，调节细胞免疫等多种功能〔见：郭　峰.红细胞免疫研究概况.中华微生物学和免疫学杂志，1995；15(3)：18〕。为探讨肺心病患者红细胞膜MDA对红细胞C3b受体的影响，我们自1995年9月～1996年4月选择住院肺心病患者进行观察，报道如下。

1　材料与方法
1.1　病例选择　正常对照组：体检健康者24例，肺心病组：

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