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四川省HIV

秦光明1　邵一鸣2　刘　刚1　苏　玲2　张灵麟1　管永军2
郑国英1　陈　钧2　马义才1　赵全壁2　Josef Kostler3　Hans Wolf3

　　摘要　使用PCR对9份采集于1996年中旬的四川省经血液途径感染的HIV-1阳性感染者外周血单个核细胞(PBMCs)样品进行扩增，获得HIV-1膜蛋白(env)基因的核酸片段，并对其C2-V3及邻区350～450个核苷酸序列进行了测定和分析。结果表明，9份样品中存在B和C两种亚型的HIV-1毒株序列，其中B亚型5份，彼此间的基因离散率为1.4%；C亚型4份，在不包括sc3号样品的情况下彼此间的基因离散率为2.6%，而sc3号样品与其它3份样品在核苷酸序列上的差异却达11.5%。与A～E参考亚型及部分B和C亚型代表株序列相比较，属四川B亚型的5个毒株与包括泰国、缅甸及中国云南德宏在内的B亚型毒株序列十分接近，基因离散率在3.6%～4.3%的范围内；属四川C亚型的4个毒株除sc3外则与代表印度毒株的C亚型共享序列及德宏C亚型毒株序列十分相似，其基因离散率均为3.1%，与非洲C亚型毒株nof的基因离散率为13.1%，而sc3号序列与印度、中国德宏和非洲C亚型HIV-1的基因离散率却在10.1%～17.2%的范围内。提示，HIV-1在四川的流行时间不长，且其B和除sc3之外的C亚型毒株的传入与流行在云南德宏州的相同亚型HIV-1毒株密切相关，而由sc3所代表的C亚型毒株则存在目前还尚不了解的其它传入来源。
　　关键词　人免疫缺陷病毒I型　亚型　基因变异

Subtype and Sequence Analysis of the C2-V3 Region of gp120 Genes among HIV-1 Strains in Sichuan Province Qin Guangming*,Shao Yiming,Liu Gang,et al.*Sichuan Sanitation and Anti-epidemic Station,Chengdu 610031
　　Abstract　Nine DNA fragments of HIV-1 env gene were amplified by PCR from uncultured peripheral blood mononuclear cells (PBMCs) obtained from HIV-1 sero-positive intravenous drug users (IDUs) in Sichuan province,in mid 1996.Their C2-V3 region (about 450 bp) was sequenced.Sequence analysis showed that there were two HIV-1 subtypes B and C,with 1.4% and 2.6% gene divergence inside each subtype,in Sichuan.The 5 subtype B strains,when comparing with sequences of several reference HIV-1 strains,were found similar to those identified in Thailand,Myanma and Yunnan province.The nucleotide sequence divergence between them was ranged from 3.6% to 4.3%. Mean while,the 4 subtype C strains except sc3,were most close to those found in India as well as in Yunnan province,and both their nucleotide sequence divergence was 3.1%.The sc3 strain belonged to subtype C but had larger genetic distance,between 10.1%-17.2%.Above results suggested a recent epidemic of both subtype HIV-1 strains in Sichuan provine and they were very close to those identified in Yunnan province.The s

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