|
钒钛磁铁矿尘对家兔肺泡巨噬细胞毒作用的体外研究 |
| |
宋永康1 陈启升1 官跃东1 张珲1 张文平1
刘进1 林群1 柯佛心2 张建新2
【摘要】 目的 探讨钒钛磁铁矿尘(钒钛尘)对肺泡巨噬细胞的毒性作用及程度。方法 运用体外细胞培养及卵壳膜盖片透射电镜法来比较钒钛尘、五氧化二钒(V2O5)、二氧化钛(TiO2)和石英(SiO2)在不同时间、剂量作用下对肺泡巨噬细胞存活率、形态和功能的影响及培养液中乳酸脱氢酶(LDH)和酸性磷酸酶(ACP)的活性变化。结果 4种粉尘均可使肺泡巨噬细胞存活率下降，LDH和ACP活性升高，形态和功能发生变化。但程度因粉尘种类而异。结论 钒钛尘对肺泡巨噬细胞的毒性作用低于V2O5和SiO2，但高于TiO2。TiO2对肺泡巨噬细胞亦有轻微的毒性作用。
【关键词】 钒 钛 粉尘
An in Vitro Study on Toxic Effect of Vanadium-Titanium-Magnetite Dust on Alveolar Macrophage in Rabbits Song Yongkang*, Chen Qisheng, Guan Yuedong, et al. *Institute for Industrial Hygiene and Protection, Panzhihua iron and Steel (Group) Co., Panzhihua, Sichuan 617023
【Abstract】 Objective To study the toxic effect of vanadium-titanium-magnetite (VTM) dust on alveolar macrophage (AM) and its hazardous extent. Methods Survival rates, morphology and function of AM were compared in rabbits exposed to dust of VTM, vanadium oxide, titanium dioxide and silica in various doses and length of time with in vitro cell culture and putamen membrane cover glass transmission electron microscopy, and changes in activities of lactic dehydrogenase (LDH) and acid phosphatase (ACP) in cell culture were measured. Results Exposure to all the four kinds of dust could lead to decrease in survival rate of AM, increase in activities of LDH and ACP in the cell culture, and changes in their morphology and function to the extent dependent on the nature of dust. Conclusion Toxic effect of exposure to VTM dust was lower than that to vanadium oxide and silica, but higher than that to titanium dioxide, which had slight toxic effect.
【Key words】 Vanadium Titaniumw Dust
攀枝花钒钛磁铁矿中含有大量的钒（15.37%）、钛（9.55%）、铁（39.15%）等金属元素及其氧化物，在开采、冶炼等过程中，工人接触密切。而有关钒钛尘对肺泡巨噬细胞(AM)的毒性作用尚未见报道。本研究运用家兔AM体外培养染尘方法，探讨该粉尘对AM的毒性作用及程度。
材料与方法
1. 粉尘:钒钛尘取自攀钢钒渣场降尘(游离SiO2含3.16%)，五氧化二钒(V2O5)、二氧化钛(TiO2)由市医药公司提供(进口)，石英(SiO2)系中国科学院提供，上述粉尘经玛瑙乳钵碾细，粒径＜5 μm达99%以上。在无菌条件下配成500 μg/ml的贮备液待用。
[1] [2] 下一页
上一个医学论文： 慢性支气管炎患者HLA
下一个医学论文： 铀矿矽肺动态分析
|
|
|
|
|
|
|
您现在的位置： 绿色健康网 >> 医学论文 >> 基础医学论文 >> 正文