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肿瘤坏死因子 对Graves病患者甲状腺细胞基因表达的影响 |
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蔡东升 刘超 覃又文 刘翠萍 蒋须勤 张忠邦 陈家伟
【摘要】 目的 研究肿瘤坏死因子α(TNFα)在有或无促甲状腺素(TSH)存在时，对甲状腺培养细胞甲状腺球蛋白(TG)、甲状腺过氧化物酶(TPO)基因转录的影响。方法 运用细胞原代培养技术和Northern Blot分子杂交技术，观察不同剂量的TNFα对甲状腺细胞TG、TPO mRNA表达的调节作用。结果 (1)TNFα在0.01U/ml时能够明显降低甲状腺细胞基础状况下TG、TPO mRNA的水平，当TNFα处于1～100U/ml时，TG、TPO mRNA的水平几乎检测不出；(2)TSH在10mU/ml时显著提高甲状腺细胞表达TG mRNA，TNFα在0.01～1U/ml剂量范围内对TSH的该作用无明显影响，但当TNFα浓度在100～1000U/ml时，对TSH的该作用表现为抑制效应。(3)TSH在10mU/ml时显著提高甲状腺细胞表达TPO mRNA，TNFα在0.01U/ml剂量范围内对TSH的该作用无明显影响，但当TNFα浓度在1～1000U/ml时，对TSH的该作用表现为抑制效应。结论 TNFα能够在基因转录水平上抑制甲状腺细胞基础合成TG、TPO mRNA，并且TNFα在较高水平时能够拮抗TSH促进TG、TPO mRNA表达的作用，但在低剂量范围内对其无明显影响。
【关键词】 肿瘤坏死因子α 甲状腺球蛋白 甲状腺过氧化物酶 甲状腺细胞 mRNA
Regulation of thyroglobulin and thyroid peroxidase gene transcription by TNFα in cultured thyrocytes from patients with Graves disease
CAI Dongsheng,LIU Chao,QIN Youwen,et al.
The First Affiliated Hospital,Nanjing Medical University,Nanjing,210029
【Abstract】 Objective To study the effects of TNFα with or without TSH on the thyroglobulin (TG) and thyroid peroxidase (TPO) gene mRNA levels. Methods Thyrocytes were isolated from thyroid tissues of patients with Graves disease and TNFα at different doses were added in the absence or presence of bovine TSH. Total RNA was extracted from thyrocytes using a single step method and it was electrophoresed and transferred to nylon filter. The filter was hybridized to TG, TPO and and GAPDH probes respectively and subjected to autoradiography. Results (1) Basal TG and TPO mRNA levels of thyrocytes were significantly decreased by TNFα of 0.01U/ml and were inhibited to undetectable level by TNFα of 1～100U/ml; (2) TNFα in the concertration of 0.01U/ml～1U/ml did not affect TSH induced TG mRNA levels while at the dose of 100～1000U/ml TNFα significantly inhibited TSH-induced TG gene transcription; (3) TNFα in the concentration [1] [2] [3] 下一页
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