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卡氏肺孢子虫肺炎肺表面活性蛋白A和D的变化 |
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瞿介明 容朝晖 何礼贤
【摘要】 目的 研究卡氏肺孢子虫肺炎(PCP)引起的肺泡表面活性蛋白A和D(SP-A、SP-D)的变化，以探讨其在PCP发病机制中的作用和治疗中的意义。方法 采用清洁级SD大鼠每周2次皮下注射醋酸可的松方法建立PCP模型为实验组，并设正常对照组，阴性对照组，细菌性肺炎组。8～12周分批处死动物，收集支气管肺泡灌洗液(BALF)，细胞计数分类；采用免疫斑点法测定BALF中SP-A、SP-D含量。结果 根据实验设计和所诱导模型分组，A组：正常对照组，n=6，健康SD大鼠；B组：阴性对照组，n=6，使用醋酸可的松(CA)8周以上未发生肺部感染的大鼠；C组：细菌性肺炎组，n=11，CA诱导8周以上发生细菌性肺炎而无其他肺部感染；D组：PCP组，n=14，CA诱导8周以上出现PCP而无其他肺部感染。BALF中SP-A含量在PCP为(45.1±22.1)μg/ml，明显高于阴性对照组的(16.2±9.9)μg/ml和细菌性肺炎组的(16.2±5.6)μg/ml(P<0.05)，同样SP-D相对含量(24 249±4 780灰度值)明显高于细菌性肺炎组(11 989±2 750灰度值)和阴性对照组(13 384±2 887灰度值)(P<0.001)。结论 PCP引起肺表面活性蛋白A和D的显著升高，且该变化较细菌性肺炎明显，应在PCP的防治中引起重视。
【关键词】卡氏肺孢子虫肺炎；支气管肺泡灌洗液；表面活性蛋白A、D
Alteration of surfactant protein A and D in bronchoalveolar lavage fluid in rats with pneumocystis carinii pneumonia
QU Jieming, RONG Zhaohui, HE Lixian
（Department of Pulmonary Medicine, Zhongshan Hospital, Institute of Respiratory Diseases, Shanghai Medical University, Shanghai 200032, China）
【Abstract】 Objective To study the alteration of surfactant protein A and D (SP-A, SP-D) resulting from pneumocystis carinii pneumonia (PCP) and investigate its implication in the pathogenesis of PCP. Methods SD rat models of PCP were induced by subcutaneous injection of 25 mg cortisone acetate, normal control and negative control as well as bacterial pneumonia group were set up for comparison. During 8～12 weeks, bronchoalveolar lavage fluid (BALF) of rats was collected. Total nucleate cells of BALF were counted and differentiated as well as the concentrations of surfactant protein A (SP-A) and surfactant protein D (SP-D) were measured by immunoblotting assay. Results The rats were divided into three immunosuppressive groups, plus a normal control group. Group A: normal control (n=6) consisted of healthy SD rats; group B: negative control (n=6) employed rats with cortisone acetate injection over 8 weeks without lung infection; [1] [2] [3] 下一页
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