5羟色胺受体可塑性在大鼠肠易激综合征中的作用

　　【摘要】 目的 探讨5羟色胺(5-HT)以及5-HT3、5-HT4受体可塑性在肠易激综合征(IBS)不同亚型发病中的作用。方法 成年雄性SD大鼠27只，随机分为IBS合并腹泻组(IBS-D)、IBS合并便秘组(IBS-C)和空白对照组。分别采用乙酸灌肠、冰水灌胃的方法制备IBS-D和IBS-C大鼠模型。用5-HT以及5-HT3、5-HT4受体抗体的免疫组化方法检测各组大鼠肌间神经丛的5-HT及其受体数量的变化。结果 ①大鼠肌间神经丛5-HT阳性肠神经元数目IBS-D组(4.37±0.88)显著高于对照组(2.99±0.41，P<0.01)，而IBS-C组(3.04±0.35)和对照组相比无差异(P>0.05);②大鼠肌间神经丛5-HT3受体免疫组化染色的灰度值各组间差异无统计学意义(F=0.082, P=0.922);③大鼠肌间神经丛5-TH4受体免疫组化染色的灰度值IBS-D组(141.98±6.96)显著低于对照组(159.90±2.55，P<0.01)，而IBS-C组(155.62±2.03)与对照组差异无统计学意义(P>0.05)。结论 大鼠肠肌间神经元内的5-HT含量及5-HT4受体数量的显著增多可能与IBS-D大鼠的发病相关。

　　【关键词】 肠易激综合征;5-HT;5-HT3受体;5-HT4受体

　　Plasticity of 5-HT receptors in the IBS subgroups of experimental rats

　　Xu Junrong, Luo Jinyan, Shang Lei, Kong Wuming

　　1. Department of Gastroenterology, the Second Affiliated Hospital,Medical School of Xi’an Jiaotong University, Xi’an 710004; 2. Department of Health Statistics,Fourth Military Medical University, Xi’an 710033, China

　　ABSTRACT: Objective To investigate the effect of 5-hydroxytryptamine (5-HT) and the plasticity of 5-HT3 and 5-HT4 receptors in the myenteric plexus on the pathogenesis of irritable bowel syndrome (IBS) subgroups. Methods Twenty-seven male rats were randomly divided into three groups: IBS with diarrhea (IBS-D) group, IBS with constipation (IBS-C) group and blank control group. IBS-D model was made in rats by intracolonic instillation with acetic acid. IBS-C model was made in rats by gastric instillation with cool water. After rats in each group were killed, a colon segment was removed for detecting enteric neuron and excitatory neurotransmitter in the myenteric plexus using 5-HT and 5-HT3, 5-HT4 receptors immunohistochemistry method. Results ① The number of 5-HT positive neurons in the myenteric plexus of IBS-D group (4.37±0.88) group was significantly higher than that in control group (2.99±0.41, P<0.01). However, there was no significant difference between IBS-C group (3.04±0.35) and the control group (P&

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