【摘要】 目的 探讨饲料加硼对过量氟摄入的大鼠骨损伤的预防作用。方法 24只4-5周龄SpragueDawley(SD)大鼠分为：对照组(喂饲基础饲料，饮蒸馏水)、过量氟组[喂饲基础饲料，饮含100mg/L F-(氟化钠221mg/L的蒸馏水)]、硼预防组(喂饲加硼饲料，饮含100mg/L F-的蒸馏水)。实验3月后测量大鼠血清氟、硼含量及血清碱性磷酸酶(alkaline phosphatase, AKP)活性、骨氟和骨总RNA含量、胫骨纵径横径和干重及骨密度(bone mineral density, BMD)，并检测大鼠股骨生物力学特性。结果 和对照组比较，过量氟组大鼠血清氟、骨氟含量和血清AKP活性显著增高;骨总RNA含量、全身骨骼平均BMD和胫骨BMD降低;胫骨纵径、横径和干重减小;股骨最大负荷减低，最大形变增加。相比过量氟组，硼预防组大鼠血清氟和骨氟含量及AKP活性明显下降;骨总RNA含量、全身骨骼平均BMD、腰椎和胫骨BMD显著增高;胫骨纵径、横径和干重，股骨最大负荷明显增加。3组大鼠血清硼含量无显著性差异。结论 大鼠摄入过量氟可出现明显的骨损伤，饲料加硼对此有一定的预防作用。
【关键词】 氟化物;骨;骨密度;骨生物力学;大鼠
Preventive effect of the supplemental dietary boron on bonedamage of rats with excess fluoride ingestion
Xu Peng, Yao Jianfeng, Cai Qiankun, Zhang Yingang, Du Xiaoyang, Guo Xiong
1. Department of Public Health, Medical School of Xian Jiaotong University, Xian 710061;
2. Xian Red Cross Hospital, Xian 710054, China
ABSTRACT: Objective To explore the preventive effect of the supplemental dietary boron on bone damage of rats with excess fluoride (EF) ingestion. Methods Twentyfour SpragueDawley rats aged 4-5 weeks old were divided into the control (C group, treated with distilled water and the elementary dietary), the excessive fluoride dose group (EF group, treated with distilled water with 100mg/L F- from 221.0g NaF per liter and the elementary dietary) and the boron prevention group (P group, treated with 100mg/L F- distilled water and the supplemental boron dietary). Three months after the experiment, fluorine, boron contents and AKP activities in serum, total RNA and fluorine contents in bone, vertical and transverse diameter and dry weight of tibias of rats were assayed, and bone biomechanics of femur and bone mineral density (BMD) were determined. Results Compared with those in the C group, fluorine contents in serum and bone, AKP activities in serum of rats in the EF group significantly increased; total RNA in bone and BMD of the bone of whole body and tibia decr
[1] [2] [3] [4] [5] 下一页
您现在的位置： 绿色健康网 >> 医学论文 >> 骨科论文 >> 正文