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补肾健脾中药对哮喘模型大鼠肺组织 |
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包照日格图 吴敦序 陈淑俊 吴昆懿 童舜华 奚蓓莉
摘要:采用放射配基结合分析法观察补肾健脾方龟芪散,补肾方补肾定喘汤和健脾方加味六君子汤扶正三方对连续激发哮喘的大鼠肺组织β-肾上腺素(β-AR)的影响。结果表明,激发后肺组织胞膜β-AR逐日下降,第7天明显低于正常对照组,激发第7天,扶正三方可明显上调哮喘大鼠肺组织β-AR密度。以上实验结果提示,扶正三方防治哮喘可能与其上调β-AR作用有关。 关键词:龟芪散/药理学;补肾定喘汤/药理学;加味六君子汤/药理学;哮喘/中药疗法 中图分类号:R245.5 文献标识码:A 文章编号:1003-9783(2000)02-0098-03
Effect of Kidney-Nourishing and Spleen-Invigorating Chinese Medicine on β-Adrenergic Receptor of Lung Tissue in Rats with Asthma
Bao Zhaorigetu (Shanghai Institute of Qigong,Shanghai,200030) Wu Dunxu (Department of Basic Theory of Traditional Chinese Medicine,Shanghai University of TCM.Shanghai,200032) Chen Shujun (Department of Basic Theory of Traditional Chinese Medicine,Shanghai University of TCM.Shanghai,200032)
Abstract:Radioligand-binding assay was applied to observe the effect of Guiqi San(GS),Bushen Dingchuan Tang(BDT) and Jiawei Liujunzi Tang(JLT)on β-Adrenergic receptor(β-AR)of lung tissue in rats with asthma induced by continuous excitation.The results showed that β-AR density in asthmatic rat group decreased day by day and was much lower than the normal control on 7th day after excitation,and GS,DT,JLT increased the decreasing level of β-AR.It is indicated that the mechanism of the above three formula for asthma is related to the increase of β-AR density in lung tissue. Key words:@Guiqi San/pharmacol.;@Bushen Dingchuan Tang/pharmacol.;Jiawei Liujunzi Tang/pharmacol.;asthma/TCD ther.
补肾健脾是中医药防治支气管哮喘的主要方法。我们在临床上长期应用补肾健脾方龟芪散、补肾方补肾定喘汤、健脾方加味六君子汤防治支气管哮喘的基础上,通过动物实验直接观察哮喘大鼠肺组织β-AR结合位点数(RT)的变化来探讨中药治疗哮喘的作用机理。
1 材料与方法
1.1. 试剂 卵蛋白,Sigma公司产品;百日咳杆菌疫苗,由上海市传染病研究所提供;丙酸倍氯米松(商品名为必可酮,以下简称必可酮),重庆葛兰素制药有限公司产品;无载体125I-NaI,Amersham公司产品;125I-心得静,我校同位素室标记,比活度为2176Ci/mmol;心得安,北京制药厂产品。 1.2 中药 龟芪散,由黄芪、人参、白术、甘草、龟板、鹿角片、巴戟和紫河车等21味中药组成。加工制粉与鼠饲料相混匀,制成饼备用;补肾定喘汤由[1] [2] 下一页 上一个医学论文: 雷公藤地上部分浸膏的抗炎 镇痛作用 下一个医学论文: 巴戟素对衰老大鼠空间学习记忆力的改善作用
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