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白细胞介素1 肿瘤坏死因子 在膝关节原发性骨关节病发病中的作用

何耀华 翁习生 邱贵兴

  【摘要】目的探讨白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)在膝关节原发性骨关节病(OA)发病中的作用。方法提取16例OA患者(OA组)、5例对照者(非OA组)的关节液,采用酶联免疫吸附法(ELISA法)测定IL-1β,生物活性法(MTT法)检测TNF-α的水平。结果在OA组关节滑液中,IL-1β的水平均显著高于对照组(P<0.001);对照组的TMF-未能测出,而OA组TNF-α的检出阳性率为25.其次,为进一步探讨滑液中上述细胞因子的产生来源,传代培养膝关节滑膜细胞并用脂多糖(LPS)刺激,OA组(20例)关节滑膜细胞自发分泌IL-1β的水平显著高于对照组(5例),P<0.05;LPS刺激的条件下,OA组和对照组中均显著升高,尤以OA组为著(P<0.001);TNF-α在OA组中仅有两例呈阳性,对照组中均匀为阴性.结论 IL-1β和TNF-α在骨关节病的发病中起重要作用.OA患者滑膜细胞高水平自发性分泌IL-1β可能与其炎症发生与发展密切相关,可能是关节滑液中IL-1β的主要来源,而滑膜细胞分泌TNF-a可能不是关节滑液中的主要来源.
  【关键词】骨关节炎 膝关节 白细胞介素1 肿瘤坏死因子

An Investigation of the Role Played by IL-1β and TNF-α on the Pathogenesis of Osteoarthrosis of the Knee

HE Yaohua,WENG Xisheng,QIU Guixing.
Department of Orthopaedics,Peking Union Medical College Hospital,Peking Union Medical College,Beijing 100730

  【Abstract】 Objective To study the relationship between IL-1β , TNF-α and pathogenesis of knee osteoarthrosis. Methods IL-1β and TNF-α were measured concurrently in synovial fluid samples from 16 patients with osteoarthrosis(OA) and from 5 normals. The determinations of IL-1β were carried out using ELISA. The determination of TNF-α was performed by bioassay. Results The concentrations of IL-1β are higher in synovial fluids from OA than those from normal (P<0.001).TNF-αconcehtrations of IL-1βare low and only 25%of the patients with OA could be ositively detectcd.To further define intra-articular sources of these cytokines,the ability of synoviocytes to produce IL-1βandTNF-αwas examined.Human synoviums isolated from normal(5 cases)and osteoarthritic joints(20 cases)spontaneouslu release Il whencultured in thd presence of serum.The le=vels of IL-1βincrease markedly when synoviocytes were stimulated by IPS either OA or normal,especially in OA.It was mot found that human synoviocyt

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