您现在的位置: 绿色健康网 >> 医学论文 >> 基础医学论文 >> 正文  

甲基汞对大脑皮层神经细胞内游离钙的影响

祝卫国 陈学敏 庄志雄 汤平涛 谢虹

摘 要:目的 研究甲基汞对急性分离的大脑皮层神经细胞内游离Ca2+水平的影响。方法 采用钙离子指示剂Fura-2双波长荧光检测技术。结果 (0.10~5.00)×10-6mol/L甲基汞可使大脑皮层神经细胞内游离Ca2+水平显著升高,且有剂量-效应关系。甲基汞升高神经细胞内游离Ca2+的作用与胞外Ca2+大量内流和胞内储Ca2+释放有关,且以胞外Ca2+内流为主。胞外Ca2+内流与N-甲基-D-天冬氨酸受体门控Ca2+通道以及电压门控Ca2+通道有关,而与电压依赖性Na+通道无关。结论 甲基汞可使神经细胞内游离Ca2+浓度异常升高,且与Ca2+通道有密切关系。
关键词:甲基汞化合物;大脑皮质;钙;神经元

Effects of methylmercury on intracellular free calcium content in cerebral cortical neurons

ZHU Weiguo,CHEN Xuemin,ZHUANG Zhixiong,et al.
(Department of Environmental Health,Tongji Medical University, Wuhan 430030, China)

Abstract:Objective This study was conducted to explore effects and potential mechanism of methylmercury on concentration of intracellular free calcium (Ca2+) in the acutely isolated cerebral cortical neurons. Methods Calcium ion indicator Fura-2 two wave-length fluorophotometry was used to measure concentration of Ca2+. Results (0.10~5.00)×10-6 mol/L of methylmercury could increase level of free intracellular Ca2+ in cerebral cortical neurons significantly (P<0.01), in a dose-response manner. Increase in intracellular free Ca2+ of neurons caused by methylmercury associated with large influx of Ca2+ outside cells and release of stored Ca2+ inside cells, especially by influx of Ca2+ outside cells. Influx of Ca2+ outside cells associated with Ca2+ channels regulated by receptor gate and potential gate, but not associated with sodium-dependent channel. Conclusion Methylmercury lead to abnormal increase of intracellular free Ca2+ in neurons, which is closedly related to Ca2+ channel.
Key words:Methylmercury compounds;Cerebral cortex;Calcium;Neurons

  有研究表明,甲基汞的神经毒性与其所致神经细胞胞内游离Ca2+水平的异常持续增高有关,但其机制尚不十分清楚[1,2]。我们采用双波长荧光光度法检测甲基汞对胞内游离Ca2+水平的影响,并对其可能的机制进行了探讨。

材料与方法

  1.试剂与仪器:氯化甲基汞(MMC、≥98%、Merck公司生产),钙离子指示剂Fura-2/AM、α-氨基-5-磷戊酸(AP5)、memantine、河豚毒素(TTX)、verapamil、Triton X-100均为Sigma公司产品,胰蛋白酶(Difco进口分装

[1] [2] 下一页


  • 上一个医学论文:

  • 下一个医学论文:
  • 相关文章
    甲基叔丁基醚对细胞周期及细胞凋亡的影
    羧甲基壳聚糖对玉米籽粒氮代谢关键酶和
    去甲基柔红霉素联合阿糖胞苷治疗急性非
    甲基硝基亚硝基胍诱发的遗传不稳定细胞
    甲基强的松龙冲击法治疗急性脊髓损伤的
    甲基强的松龙冲击法治疗急性脊髓损伤的
    宫颈癌组织中抑癌基因Syk启动子甲基化
    白介素6拮抗N甲基D天门冬氨酸诱导的神
    Runx3基因甲基化与胃癌发生和转移的关
    抗氧化维生素减轻高脂饮食兔动脉粥样硬
    MTT比色法中应采用分析纯二甲基亚砜作
    甲基硝基亚硝胍处理的vero细胞中磷酸化
    用甲基纤维素半固体培养基筛选杂交瘤细
    Copyright © 2006-2012 绿色健康网(www.gio.org.cn) All Rights Reserved.
    声明:网站信息仅供参考,不能作为诊断及医疗的依据。