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端粒酶活性与慢性粒细胞白血病病程关系的研究 |
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倪晓华 毕富勇 曹根涛
［摘 要］ 目的：通过对慢性粒细胞白血病(CML)各期患者骨髓单个核细胞端粒酶活性的检测，以了解端粒酶活性与CML病程的关系。方法：采用聚合酶链反应-酶标记免疫吸附分析法(PCR-ELISA)测定端粒酶活性。结果：正常骨髓组端粒酶活性为9.85±0.68，CML慢性期为24.48±12.73，明显高于正常骨髓组（P＜0.05），加速期为76.76±21.84，急变期为90.62±25.41，两者均明显高于慢性期组（P＜0.001，P＜0.001）且本身两组间无显著差异（P＞0.05），慢性期治疗后为18.12±6.27，仍高于正常骨髓组（P＜0.05）。结论：CML各期端粒酶活性均显著高于正常骨髓组，端粒酶的异常激活表明CML患者进入了加速期，端粒酶活性可作为CML检测和预后的一个有用的新指标。
［主题词］ 端粒；骨髓；白血病， 髓性，慢性
［中图分类号］ R733.7 ［文献标识］ A
［文章编号］ 1000-4718(2000)05-0454-03
Relation between telomerase activity and chronic myelocytic leukemia course
NI Xiao-hua， BI Fu-yong,
（Dept. of Biochemistry Wanan Medical College， Wuhu 241001， China)
CAO Gen-tao
(Shanghai Institute of Cell Biology, the Chinese Acadmy of Science, Shanghai 200031, China)
［Abstract］ AIM： Studying the relation between telomerase activity and chronic myelocytic leukemia(CML) courses by detect telomerase activity(TMA) of mononuclear cells of bone marrow samples in different CML phases. METHOD: Using telomerase PCR-ELISA method. RESULTS: Telomerase activity of normal bone marrow is 9.85±0.68, CML chronic phase is 24.48±12.73 which is significantly higher than that of normal bone marrow(P＜0.05）. CML accelerated phase TMA is 76.76±21.84 and blast crisis is 90.62±25.41, both are significantly higher than that of chronic phase(P＜0.001, P＜0.001）but there is no significant difference between accelerated phase and blast crisis(P＞0.05）, at remisson TMA is 18.12±6.27 which is still higher than normal group(P＜0.05).CONCLUTIONS: Telomerase activity in different phases of CML is significantly higher than normal bone marrow group. When telomerase is extremely activated , it means that CML patients were entering the accelerated phase. Telomerase activity might be used as an useful new marker in detecting and curing CML.
［MeSH］ Telomere; Bone marrow; Leukmia, [1] [2] [3] 下一页
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