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一株新轮状病毒引起河北省石家庄市成人腹泻爆发流行 |
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杨红彦1 陈淑芬2 纪绍忠1 孟宗达2 康志刚3 张 忠2 齐顺祥2
摘要 1997年4月10～28日，在河北省石家庄市某高校发生一起成人急性腹泻的爆发流行，累积1 000多名大学生发病。经不连续聚丙烯酰胺凝胶(PAGE)电泳检查腹泻病人粪便标本核酸图谱一致呈4-2-1-1-1-1-1排列的新轮状病毒(新RV)14份，阳性率占47%(共检标本30份)，且未见其它带型的轮状病毒。提示该核酸图谱的轮状病毒是此次成人急性腹泻爆发流行的主要病原。用已知ADRV第5及第9基因片段末端引物进行逆转录-聚合酶链反应(RT-PCR)，结果阳性对照ADRV的扩增结果都为阳性，而新RV的扩增结果都为阴性。这些结果表明该新RV不属于B组轮状病毒，是与成人腹泻轮状病毒(ADRV)完全不同的新RV。非ADRV的轮状病毒引起成人腹泻爆发流行在河北省尚属首次。
关键词 成人腹泻 新轮状病毒 RT-PCR
A Novel Rotavirus Causing Large Scale of Adult Diarrhea in Shi Jiazhuang Yang Hongyan*, Chen Shufen, Ji Shaozhong,et al. *The Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing,102206
Abstract An outbreak of adult diarrhea causing more than 1 000 hospitalized cases among graduates in a university in Shi Jiazhuang city (Hebei province),occurred in 10-28,April,1997. The non-bacterial pathogenic agent is confirmed as Rotavirus first by PAGE. Forteen samples have been demonstrated sharing the same nucleic acid electrophoresis pattern of 4-2-1-1-1-1-1. The positive rate was 47% (thirty samples have been checked) with no other RV pattern. Dada showed that RV with this kind of nucleic acid electrophoresis pattern were responsible for the outbreaks acute diarrhea in adults. The novel RV and known ADRVwas RT-PCR with end-primers 5, 9 of typical ADRV, the positive control typical ADRV s results are positive, the novel RV s are negative. Experimental results showed that the novel RV did not belong to group B RV, totally different from all the known ARDV. This case was the first reported one in Hebei province with an outbreak of adult diarrhea not by any known ADRV.
Key words Adult diarrhea Novel Rotavirus RT-PCR
引起人类腹泻的病毒有轮状病毒(Rotavirus,RV)、诺瓦克病毒、杯状病毒、星状病毒、冠状病毒、肠道腺病毒等多种病毒，迄今为止已报告的轮状病毒有七个组，其中人畜共患的轮状病毒只有A、B、C三组，其他四个组只引起动物的传染性腹泻。但造成人类严重危害的主要是A、B两组轮状病毒。A组轮状病毒致婴幼儿腹泻，目前已根据它的中和抗原VP7的多态性分为至少十四个血清型，国际上已针对婴幼儿重型腹泻的四个血清型病毒研制并开发了疫苗，并准备把该疫苗纳入扩大免疫计划。成人腹泻轮状病毒(ADRV)是我国学者洪涛1983年［1］发现的B[1] [2] 下一页
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