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应用逆转录聚合酶链反应检测原发性肝癌患者外周血肿瘤细胞 |
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罗元辉 房殿春 鲁荣 刘为纹 门荣甫
【摘要】 目的 寻求检测循环血中肝癌细胞的灵敏方法。方法 采用逆转录聚合酶链反应(RT-PCR)技术，对肝癌患者外周血有核细胞人甲胎蛋白mRNA(AFP mRNA)进行检测。结果 31例原发性肝癌中有15例检出AFP mRNA，阳性率为48.4%。良性肝病、肝转移癌患者和健康对照组均为阴性。外周血细胞AFP mRNA的存在与肝内转移、门静脉癌栓和远处转移密切相关。结论 外周血细胞AFP mRNA是反映原发性肝癌患者有无血行播散的重要标志，RT-PCR技术是检测循环血中原发性肝细胞癌的灵敏方法。
【关键词】 癌，肝细胞 聚合酶链反应 甲胎蛋白类
Detection of hepatocellular carcinoma cells in peripheral venous blood with reverse transcriptase-polymerase chain reaction Luo Yuanhui, Fang Dianchun, Lu Rong, et al. Department of Gastroenterology, Southwest Hospital, The Third Military Medical University, Chongqing 630038
【Abstract】 Objective To identify circulating hepatocellular carcinoma (HCC) cells. Methods We detected the expression of human α-fetoprotein mRNA (AFP mRNA) in the nucleated cell component of peripheral blood from patients with HCC using nest reverse transcription-polymerase chain reaction (RT-PCR). Results AFP mRNA was positive in 15 of 31 cases (48.4%) of HCC, but negative in peripheral blood from those with benign liver diseases, other tumors metastatic to liver and normal healthy volunteers. The presence of AFP mRNA in blood seemed to be correlated with the presence of intrahepatic metastasis, portal vein thrombosis and/or distant metastasis. Conclusion These findings indicate that the presence of AFP mRNA in peripheral blood may be an indicator of circulating HCC cells, which might predict hematogenous metastatic spreading of tumor cells in patients with HCC, and that RT-PCR is a very sensitive method for detecting circulating HCC cells.
【Key words】 Carcinoma, hepatocellular Polymerase chain reaction Alpha fetoprotein
大量临床资料表明，肿瘤的转移和复发是影响肿瘤患者预后的重要因素［1］。血行播散可能是原发性肝癌转移和复发的主要原因［2］，因此，及时发现外周血的肿瘤细胞不仅对判断肿瘤的转移、复发具有重要价值，而且对指导临床制定治疗措施亦有重要意义。但由于肿瘤脱落至循环中的细胞数很少，现有的细胞形态学及免疫细胞化学技术敏感性较低，使其在检测外周血中少数肿瘤细胞方面的应用受到限制。应用逆转录聚合酶链反应(RT-PCR)技术，扩增肿瘤患者外周血肿瘤细胞“标志性”靶RNA来证实血中肿瘤细胞的存在，是新近发展起来的检测外周血和骨髓中肿瘤细胞的新技术［3］。由于其灵敏度高、特异性强及简便快速等[1] [2] 下一页
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